FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_SBP-bZIP53_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_SBP-bZIP53_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13469706
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG157930.11724829034872773No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG27250.09.1201321
CAGTACG53600.08.29289770
TGTCAGT105900.07.23794970
TGTAGCC91050.07.034585561
GTCAGTA92200.06.75703970
TCGTGTT85250.06.73312770
GTGTAGC97750.06.58822560
AGTACGT61200.06.576786570
TCAGTAC78250.06.485612470
GGTGTAG103050.06.351275459
AAAGTCC100250.06.284279368
CGACTCC128500.05.992207510
GTGTCAG137950.05.93692469
AGAGCGG30800.05.90908270
AGTGTGT184300.05.906121770
GTAGCCA117800.05.88284362
GTGTGTC146400.05.833324470
AGAGTGT218050.05.77848270
CACTAAC167850.05.630017821
AGGGAAA366750.05.592356770
Produced by FastQC (version 0.11.5)