FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_SBP-ColSPL16_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_SBP-ColSPL16_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091504
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46560.22261492208477726No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4300.043.1394776
GGACTGT4850.038.9690177
GACTGTA5600.030.6249588
GCGGACT2950.024.915225
TAGTCCG3000.024.49996611
ACTGTAG7250.024.1378979
TCGGACT4250.023.0587925
CGGACTA3200.022.9687186
CTAGTCC3650.021.09586110
CTGTAGA8850.020.56494310
CAGTCCG4300.020.3488127
GGACTAG4350.018.5057227
TACAGTC5850.016.75211325
ACGGACT7900.015.9493465
GACTAGT4850.015.87626658
ACAGTCC5750.015.82606526
AACGGAC3552.1827873E-1115.7746264
TGCGGAC1605.164691E-415.3124794
GACGGAC2101.5828033E-514.999984
AGCGGAC2253.0354651E-513.9999814
Produced by FastQC (version 0.11.5)