FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_SBP-C24SPL16_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_SBP-C24SPL16_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115942
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27410.24562208430187232No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG2500.046.1999136
GGACTGT2900.039.8275077
CGGACTC1700.032.9411136
GCGGACT1550.031.612845
GACTGTA3350.031.3432248
TCGGACT1900.031.315735
ACTGTAG4000.026.249959
TAGTCCG1851.4551915E-1124.59454711
CGGACTA2150.024.4185586
GACGGAC1301.6075683E-724.2307224
ACGGACT4450.023.595465
CCCCGCT800.002667127921.87495837
CAGTCCG3250.021.5384227
ACAGTCC3750.020.53329326
CCGGACT1751.2654345E-719.9999625
TGCGGAC1408.251036E-619.9999624
CTGTAGA5550.018.91888210
CTAGTCC2508.6583896E-1018.19996610
AACGGAC1752.7960868E-617.9999664
TACAGTC4300.017.90694225
Produced by FastQC (version 0.11.5)