FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_SBP-C24SPL14_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_SBP-C24SPL14_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086227
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22870.21054530958998444No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG3250.049.538436
CGGACTA2000.036.7499776
GGACTGT4400.034.9999777
TAGTCCG2250.034.22220211
GACTGTA3750.033.599988
TCGGACT2700.032.4073875
GCGGACT1600.030.6249795
GGACTAG2550.030.196067
CCGGACT1551.8189894E-1229.3548185
ACTGTAG4600.028.9130259
CTAGTCC2650.027.7358310
CGGACTC1800.027.2222046
CTGTAGA5050.025.64354710
AACGGAC2100.024.9999854
CAGTCCG3400.024.70586627
ACGGACT4800.024.0624855
GTCCGCA901.9287867E-423.33331913
GACTAGT3400.022.6470438
ACTAGTC3850.019.9999879
AGCGGAC1408.250612E-619.9999874
Produced by FastQC (version 0.11.5)