FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_SBP-C24SPL11_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_SBP-C24SPL11_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804293
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33870.18771895695433058No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG2050.037.5609446
CAGTCCG2306.0863385E-918.26085527
GGACTGT3750.017.733327
TCGGACT2302.2176646E-615.2173795
GACTGTA4651.200533E-1012.795698
ACTGTAG5451.0913936E-1112.2018259
AACGGAC2356.849758E-411.9148854
ACGGACT5004.0563464E-1011.8999915
ACAGTCC4004.1964267E-711.37499126
CGGACTC3104.8014255E-511.2903146
CTGTAGA7352.3646862E-119.99999210
TCGTGTT10750.09.76743370
TACAGTC4451.836827E-59.43819425
CTACAGT4703.4999084E-58.93616324
TCTACAG5957.3079846E-68.23528823
AGGGGGG4450.00180192687.86559771
GTAGAAG9151.5410478E-87.65026712
GAGTGTT31550.07.32170670
AGAGTGT34600.06.77745169
TGTTAAG25850.06.634424769
Produced by FastQC (version 0.11.5)