FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_HALO-bZIP9_SBP-bZIP53_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_HALO-bZIP9_SBP-bZIP53_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14685918
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG216040.14710690880883306No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTGT55400.09.79243370
TCGCCTG69550.09.66212870
CCTGTGT60700.08.41846470
AGGGGGG30100.08.3728741
CGGACTG16400.08.32315356
TTCGCCT85800.07.95455769
GCCTGTG66950.07.89396270
CTTCGCC115150.07.72037670
GTCTTCG146100.07.47434470
CGAGCGC20000.07.000010564
GTGAGCG23500.06.8510525
GTCCCGA24050.06.839903416
CGGCTAG17550.06.78063767
GGCTAGG21050.06.65084168
TCGTGTT89050.06.56373870
TCTTCGC136600.06.533684369
GATCCGC30550.06.53037642
GCGAGGT23800.06.470575329
CCCGCGT15904.0017767E-116.383656565
AGTGTCT212400.06.261779370
Produced by FastQC (version 0.11.5)