FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_HALO-bZIP63_SBP-bZIP53_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_HALO-bZIP63_SBP-bZIP53_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18010881
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG220790.12258700726521929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG31250.08.8488681
TGTAGCC122500.06.885701761
AGTGTAG176150.06.75559469
GTGTAGC126550.06.58236760
GGTGTAG134750.06.4675259
AGTCAGC138400.06.44933271
TAGGCTA93000.06.247300670
GTGTAGG548600.06.124669670
GTAGGCT94850.06.0885569
AGGCTAT87300.06.05382670
GAGTGTA183650.06.04137270
TGTAGGC120600.06.036473369
AAAGTCC134050.05.90077268
AGTCACG55250.05.76527171
AGTCACA115700.05.74818661
CGACTCC154200.05.74253210
CATGGTG159150.05.739857756
AGTCATA357800.05.7328151
AGTCAGA140550.05.70315651
GCTATAG70600.05.65154869
Produced by FastQC (version 0.11.5)