FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_HALO-bZIP53_pIXSBP_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_HALO-bZIP53_pIXSBP_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8774668
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100680.11473938387184562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG16400.09.177551
CGGGGGC7201.7158527E-88.749989545
GGGGCCT7755.173624E-77.677409647
GGGCCTC8856.960909E-87.514115348
TACGTCC41050.07.503035570
GTACGTC43250.07.20230370
CCCGCGC8552.4454548E-66.95905654
CCGCGCT8602.679828E-66.91859655
AGTCATG53900.06.6888371
AGTCACA57650.06.67875531
AGTCAGC78050.06.63729331
AGTCACC43900.06.5380991
CGCCCGT10208.2666884E-76.519599446
ACGTCCG11853.7898644E-86.49788270
GAGTGTA83000.06.409630370
GGGGGCC10104.8718666E-66.23761646
ATACGTC26550.06.0640233
GTCCGGA10407.83685E-66.057684430
GTCCTGG35300.06.04815170
GCCCGTC9306.2072024E-56.021497747
Produced by FastQC (version 0.11.5)