FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_HALO-bZIP25_SBP-bZIP53_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_HALO-bZIP25_SBP-bZIP53_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5870404
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95380.16247604083126135No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG8350.030.5987426
GGACTGT13200.019.090877
CAGTCCG8350.015.92811227
GACTGTA14350.015.6097268
ACTGTAG16500.013.363619
AACGGAC8200.012.8048534
TCGGACT9050.012.7624065
ACAGTCC12150.011.81067726
CGGACTC7500.011.6666446
ACGGACT19600.011.2499785
CCGGACT6452.0008883E-1110.8526935
CTGTAGA20850.010.74338310
CTACAGT15800.010.63289124
TACAGTC14250.010.56138325
GCGGACT5906.7399014E-89.4915075
AGGGGGG13600.09.2658721
CGGACTA5704.1548446E-79.2105086
GCCGTGT15850.09.0536169
AGCCGTG22800.08.90349168
AGAGCCG33600.08.8541570
Produced by FastQC (version 0.11.5)