FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_HALO-bZIP10_SBP-bZIP53_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_HALO-bZIP10_SBP-bZIP53_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13476933
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG189250.14042512491529044No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG22550.09.15819551
AGCCCGC12150.08.35389763
GGATAGG77950.08.30659570
TAGGGTG61800.07.928790670
ATAGGGT69050.07.65386370
AGGGTGT53500.07.588773770
GGGTGTA41550.07.328509368
GTCCCGA20750.07.084326716
CGGACTG14705.456968E-126.90475136
GGCGCTT20800.06.89961671
GTGAGCG19400.06.8556625
CGGCTAG14857.2759576E-126.83500767
TGTCCCG24100.06.82571615
GATAGGG89600.06.718740570
AGGATAG105950.06.705983670
CCCGCGT12703.2050593E-96.614163465
CTAGGCT25550.06.57533269
AGTGTAG181000.06.555238769
CGGGCAG12854.125468E-96.536955456
TAGGCTG25800.06.51161810
Produced by FastQC (version 0.11.5)