FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_HALO-CCA1_SBP-LHY1_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_HALO-CCA1_SBP-LHY1_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9368667
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG333000.3554401069010138No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG10200.019.9019176
TGTGCCG40500.017.02465270
GCGTCGG29500.012.45759970
GGACTGT16950.012.1828627
CAGTCCG10350.011.15939527
TCGTGTT127700.010.77131570
TGCCTCG26000.010.36536170
AGAGCGG48750.09.7640870
CTATGTG70400.09.39628670
GACTGTA20100.09.2288358
GTGCCTG17900.09.18992370
GCGGACT6806.346636E-88.749985
CCTCTAT84450.08.744870
ACTGTAG21350.08.6885059
GTAGGGG30400.08.63484870
GCCTCTA85750.08.53059269
TAGTCCG10303.6379788E-128.49512611
AGTGTGG65200.08.37421470
GTGCCTC118250.08.22831269
GTGTGCC188700.08.12398870
Produced by FastQC (version 0.11.5)