FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_ColSPL9_col_B_n3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_ColSPL9_col_B_n3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223907
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11150.49797460552818806No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTC1500.070.06
CTAGCGG150.00222255970.02
AACGGAC1900.068.157894
CGGACTG7200.067.083336
TCGGACT3300.066.8181845
GGACTGT7050.066.5248267
ACGGACT4500.066.1111155
ACCGGAC700.065.04
CGGACTA2600.064.615396
CCGGACT2000.063.0000045
GCGGACT2250.062.222225
GACTGTA7150.061.678328
ACTGTAG7200.060.2777759
AGCGGAC650.059.2307744
CACGGAC1200.058.3333324
GGACTAG2700.058.3333327
ACTAGTC2650.058.113219
CTGTAGA7600.058.02631410
CCCGGAC800.056.8754
GGCGGAC509.767973E-1056.0000044
Produced by FastQC (version 0.11.5)