FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_ColSPL9_col_B_n2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_ColSPL9_col_B_n2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381534
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10700.2804468278056477No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCCC150.002223828369.9994715
CGGACTG6650.066.315296
GGACTGT6700.065.298027
TGCGGAC650.064.61494
GCGGACT1800.062.2217565
GACTGTA6850.060.8024678
TCGGACT2800.059.9995545
CCCGGAC553.6379788E-1157.27234
ACGGACT5000.056.6995745
CGCGGAC700.054.9995884
ACTGTAG7700.054.0905049
CGGACTA2100.053.3329356
GCCCCGG200.00694870252.49960735
CACGGAC1400.052.4996074
GCTGCGA200.00694870252.49960750
AACGGAC1400.052.4996074
CGGACTC2250.051.3329476
CCGGACT2950.049.8301355
CTGTAGA8300.049.75866310
GACGGAC1150.048.6952864
Produced by FastQC (version 0.11.5)