FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_ColSPL9_col_B_n1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_ColSPL9_col_B_n1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484014
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21770.44978037825352163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG3100.056.451536
GGACTGT3100.055.32257
GACTGTA2950.054.576198
CTCGCTA200.00694965852.4999246
CGCGGAC352.0856873E-549.9999244
ACTGTAG3500.045.9999359
GCGGACT1450.043.4482085
CTGTAGA4100.040.12189510
CCGGACT1350.038.8888365
TGCGGAC459.194088E-538.888834
TAGTCCG1300.037.69225311
GACGGAC753.535024E-837.333284
CGGACTA1350.036.2962466
CTACAGT2650.034.33957324
GTAGAAG4900.034.28566412
TCGGACT1350.033.7036555
ACGGACT3650.033.5615925
CGGACTC1109.0403773E-1031.8181326
TACAGTC2650.031.69806725
GGACTAG1550.031.6128567
Produced by FastQC (version 0.11.5)