FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_ColSPL8_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_ColSPL8_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3442209
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64890.18851266730172397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG8100.030.678956
GGACTGT9750.024.4102087
CCGGACT4250.022.2352495
ACTGTAG11150.019.4618439
GACTGTA11300.019.20358
TCGGACT5950.018.2352565
CGGACTA5100.017.1568266
CAGTCCG6650.016.84207227
GCGGACT4500.015.5555245
CGGACTC5450.013.4862116
CTGTAGA16600.013.07226210
GGACTAG6750.012.9629357
ACGGACT14850.012.9629355
TAGTCCG6800.011.8382111
ACAGTCC9850.011.37053426
CTAGTCC6805.456968E-1210.80880110
CTACAGT10850.010.64513924
GTAGAAG19900.010.37686312
TCTACAG13550.010.33208223
TGGTCGC4501.9137206E-610.11109155
Produced by FastQC (version 0.11.5)