FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_ColSPL7_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_ColSPL7_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2867669
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39060.13620818860196207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG3850.034.545436
GGACTGT5850.022.7350257
CAGTCCG3700.019.86485127
GCGGACT2552.1800588E-816.4705755
ACAGTCC5850.014.35896326
GACTGTA9100.014.230768
CCGCCTT5050.013.86137656
ACGGACT6650.013.1578855
CGCCTTA4902.1827873E-1112.85713357
CCGAGGT5501.2732926E-1112.090947
GTATCCG5903.6379788E-1211.86439852
AACGGAC4155.3123586E-811.80721954
ATCCGCC5652.0008883E-1111.76990354
ACTGTAG11150.011.6143419
GGGTAGA6251.0913936E-1111.19999266
GGTATCC6653.8198777E-1110.52630851
CGGACTA3004.4425856E-410.4999916
CGAGGTA6704.3655746E-1110.44775348
CTGTAGA11450.010.39300510
CGTTTCG6754.9112714E-1110.37036228
Produced by FastQC (version 0.11.5)