FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_ColSPL6_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_ColSPL6_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251481
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42250.33760001150636726No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG2950.049.8304526
CGGACTA2200.039.7726826
CCGGACT1650.038.1817745
GGACTGT4150.034.5782747
GCGGACT2100.029.9999665
GACTGTA4500.027.2221898
ACTGTAG4650.027.0967439
CCCGGAC1207.4111085E-826.249974
TAGTCCG3200.026.2499711
CTAGTCC3300.024.39391110
CAGTCCG3750.024.26663827
TCGGACT3350.024.0298255
CGGACTC2350.023.829766
GGACTAG3250.023.6922827
ACAGTCC4100.023.04875226
ACGGACT4550.022.3076675
TGCGGAC952.7816193E-422.1052384
CTGTAGA6400.021.87497310
AACGGAC1957.8034645E-1021.5384394
TACAGTC4600.021.30432325
Produced by FastQC (version 0.11.5)