FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_ColSPL5_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_ColSPL5_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7541334
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCC7100.012.32392869
CGTCCCC7900.011.07593568
CTAACGC9950.010.20099364
AGGACGC6201.4142643E-89.59676355
CGCCTGG5502.4754627E-79.54544459
TCGGTAG13700.09.45254352
TCCCCCG9051.8189894E-129.28175670
CGATAGG15400.08.636353556
CGGGTAG10353.6379788E-128.4540964
CGCACGC5805.1945954E-68.44826627
GGCACTG16100.08.04346867
CCGATAG16650.07.98797855
GGACGCC6653.7978516E-67.89472756
CCCTGCG10758.367351E-117.81394453
TGCCGGG4100.0076099427.682917626
CCGGCAG11901.2732926E-117.64704936
GACGCCT7109.601694E-67.394356757
CCGTCCC11604.4929038E-107.241370767
GGACTGT11907.8398443E-107.05881457
GCACGCG6451.7651294E-47.05425528
Produced by FastQC (version 0.11.5)