FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_ColSPL4_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_ColSPL4_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7533724
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCC6400.012.57809969
CGATAGG14800.09.93241256
TGCCGGG3854.2871095E-49.09089126
CCGATAG16550.08.24771955
CGTCCCC9151.8262654E-98.03277168
GCGGGGA5502.1907984E-47.636348748
CCCCCGG5652.9879872E-47.433613369
ATAGGAG23400.07.179472458
ACTGTAG17150.07.14284329
TCCCGGG8057.455281E-66.95650819
CCGGGCC6702.8137877E-46.79103121
ATGGGGC5200.0082407486.730756350
CGTTCGC7904.2811243E-56.645556470
CGGGCCT7401.311961E-46.621608322
TCCCCCG9652.313136E-66.52848570
GATAGGG17750.06.50702970
CGGTAGG6500.00147768886.4615264
GGACTGT16803.6379788E-126.4583217
CCCTGCG7601.8560492E-46.447355353
GGAGTAG24150.06.231871661
Produced by FastQC (version 0.11.5)