FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_ColSPL3_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_ColSPL3_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2552008
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45030.17644929012761718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG6800.022.1323036
GGACTGT7050.019.361667
TAGGCAC3156.184564E-1116.66662858
CCGACTG3800.016.5789118
CTAGGCG4500.016.33329653
ACTGTAG7550.015.7615559
GGCACTG3401.9645086E-1015.44114260
GACTGTA7500.015.3999668
CTAACGC3653.45608E-1115.3424364
GGTCCCT4400.015.11360350
TCGGACT5600.014.3749675
CCCTGCG4151.8189894E-1114.33731853
GCGCGAG4650.014.301043530
TAGGCGG5100.013.7254654
AGCGCGA4701.0913936E-1113.40422529
TCGGGAC4506.91216E-1113.22219243
CGACTGT5103.6379788E-1213.03918719
CAGTCCG5800.012.67238627
CTTCGGG5255.456968E-1212.66663841
AGGCACT4153.916284E-912.65057559
Produced by FastQC (version 0.11.5)