FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_ColSPL1_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_ColSPL1_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4455614
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49170.11035516092731551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG7850.017.834366
CCCGGGG3051.2141754E-814.91800437
GACGCCC3354.1700332E-813.58206433
CGCCCGG3751.2929377E-813.06664135
GGCCCCG2305.7198893E-412.17388913
ACGCCCG3852.5605368E-711.81815834
GGACTGT11700.011.6666447
CGCCTGT6301.8189894E-1211.66664435
CCGACTG4257.397102E-811.52938918
GCGGACT4752.8965587E-811.052615
TCGGACT7650.010.98037055
TCGCCTG6805.456968E-1210.80880334
TCGAGCG3004.4436697E-410.4999830
CCGTCCC6058.56744E-1010.41320370
TCGCCCG3401.2277027E-410.29409742
GCCCGGG4451.6588492E-610.224736
CCCAGTC4854.5701017E-710.10307465
CTAGGTG4854.5701017E-710.10307457
GCGTGCG5251.2567398E-79.99998141
CCCTGCG3551.8984207E-49.85913653
Produced by FastQC (version 0.11.5)