FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_ColSPL16_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_ColSPL16_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121323
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7420.6115905475466317No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAC150.002220096570.000014
GGACCGC150.002220096570.0000115
CGGACTG1250.067.26
GGACTGT1200.064.166677
GACTGTA1300.059.2307668
GGTCGCC308.335177E-658.33333656
GCGGACT553.45608E-1157.2727245
TCTACAG1200.052.50000423
TACAGTC1200.052.50000425
GCCTTGC200.006937131352.557
TGCCCCC200.006937131352.561
CTCGTGA200.006937131352.54
CGACGTG200.006937131352.539
TGGACCG200.006937131352.514
GACCGCA200.006937131352.516
CGATTGC200.006937131352.557
AGCGGAC407.42848E-752.54
TCGGACT800.052.55
ACGGACT407.42848E-752.55
CCATAGG200.006937131352.539
Produced by FastQC (version 0.11.5)