FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_ColSPL15_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_ColSPL15_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1958186
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50700.25891309610016616No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG5750.044.434756
GGACTGT6750.038.3703427
ACTGTAG7600.032.236829
CCGGACT3750.029.8666445
GACTGTA8450.029.8224668
TCGGACT3700.026.486475
AACGGAC3450.026.3767934
CTGTAGA9350.026.20319210
CGGACTA3250.025.8461366
TAGTCCG3050.024.09834311
CAGTCCG5850.023.33331527
GCGGACT2750.022.9090755
GTAGAAG11100.022.70268412
ACGGACT7650.021.0457365
CTACAGT7050.020.85104824
CGGACTC3250.020.4615256
ACCGGAC2402.3646862E-1120.4166534
GGACTAG4450.019.6629097
AGTCCGA4000.019.24998528
ACAGTCC7050.018.86523426
Produced by FastQC (version 0.11.5)