FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_ColSPL13_col_B_n2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_ColSPL13_col_B_n2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5314962
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115420.21716053661343204No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG5750.026.7825836
GGTCGCC4250.017.294156
CGCCGTA4700.014.89360259
GGACTGT10450.014.40197
CAGTCCG6200.012.98385927
GACTGTA12000.012.5416568
TCGGACT8250.011.8787775
ACTGTAG13150.011.7110169
GCCGTAT6650.011.57893760
TGGTCGC6603.274181E-1110.60605155
ACGTCCG6351.9826984E-99.92124970
ACAGTCC8600.09.76743226
CCGGACT5957.7226105E-78.8235215
CCGTATC8001.2569217E-98.74999261
CGTCCGG5603.2356565E-68.74999165
CTCGGTG8851.03682396E-108.70055750
TAGTCCG5704.108735E-68.59648311
CCGGGTG4502.138418E-48.55554868
GTCGCCG9001.4733814E-108.55554857
GTCCGGG5502.438296E-58.27271966
Produced by FastQC (version 0.11.5)