FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_ColSPL13_col_B_n1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_ColSPL13_col_B_n1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995586
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55460.18513906794864177No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG3500.030.9998466
CAGTCCG3650.018.21908827
GGACTGT6200.017.4999127
GACTGTA6950.015.1078388
GGTCGCC3102.3882785E-713.5483256
ACTGTAG7800.013.0127559
TGGTCGC3651.7263865E-611.50679255
CGCCGTA2802.3466104E-411.24994459
TCGGACT4259.1850234E-710.705835
CTGTAGA9950.010.55271110
ACAGTCC6402.2682798E-99.84370126
CTACAGT7807.8216544E-119.4230324
TCGGTGG4956.448221E-69.19202651
TACAGTC7655.2168616E-98.69276725
ACGGACT9051.6370905E-108.50824455
GCCGTAT4000.00609784857.874960460
AACGGAC4905.349409E-47.85710334
GTCGCCG5006.641064E-47.69996157
GTAGAAG14700.07.61900912
TCTACAG12051.02591E-96.97091923
Produced by FastQC (version 0.11.5)