FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_ColSPL12_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_ColSPL12_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727522
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45500.16681808616025828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4750.035.3683786
GGACTGT6600.025.4545127
TCGGACT4550.021.5384335
ACTGTAG7300.021.0958619
GACTGTA8800.017.4999778
CAGTCCG5650.014.86723727
CTGTAGA10950.014.38354310
GCGGACT3405.0606104E-813.3823365
CCGGACT2356.851768E-411.9148785
CGGACTA4701.9463187E-911.9148786
TAGTCCG5054.783942E-1011.78216411
GTAGAAG15150.011.5511412
CTACAGT9100.011.53844624
ACAGTCC8050.010.86955226
GGACTAG4551.8984792E-710.7692177
CTAACGC2600.001596707610.76921764
GGTCGCC2300.00789345510.6521656
TCTACAG10450.010.04783323
GCCTGTG7807.2759576E-129.87178270
GGCACTG3955.1646624E-59.74682260
Produced by FastQC (version 0.11.5)