FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_ColSPL11_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_ColSPL11_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469452
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22530.47992126990618844No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCC150.002224095469.9998911
CGGACTG4950.065.05046
GGACTGT5300.058.1131177
GCGGACT1450.057.9309465
CCGGACT1800.054.444365
TCGGACT2600.053.846075
ACTGTAG5350.053.644789
GACTGTA5500.053.4544648
AACGGAC1550.051.9354024
CGGACTC1300.051.1537676
CTGTAGA5700.050.96483610
ACGGACT3600.048.6110345
TAGTCCG1200.043.74993511
CGGACTA1550.040.64516
CTAGTCC1300.040.38455210
GTCCGAC350.001255019639.9999429
GTAGAAG7300.039.7944612
AGCGGAC801.4206307E-939.374944
CGCGGAC556.690814E-638.181764
CCCGGAC556.690814E-638.181764
Produced by FastQC (version 0.11.5)