FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_ColSPL10_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_ColSPL10_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246336
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46830.3757413731128684No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG3650.055.6164056
GGACTGT4250.045.294097
TAGTCCG2300.038.04345711
GACTGTA5200.036.3461348
CCGGACT2350.034.25535
CGGACTA2500.033.599986
ACTGTAG5750.032.869559
AACGGAC2450.031.4285534
GCGGACT1800.031.1110925
TCGGACT3050.030.9835915
ACGGACT4950.030.4040245
CTGTAGA6400.029.53123310
GGACTAG2950.028.4745627
CTAGTCC2950.028.47456210
CGGACTC2350.028.2978556
CAGTCCG4150.026.98793627
CGCGGAC851.309112E-424.7058684
CCCGGAC1001.3988925E-524.4999874
CGGACAG750.001833123423.3333260
TGGTCGC1102.9237286E-522.27271555
Produced by FastQC (version 0.11.5)