FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_C24SPL9_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_C24SPL9_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10033897
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCG13550.010.33208370
GCCTCTA34400.07.52905570
GTGTGCC58500.07.239302669
AGCGCTG5955.410615E-47.058809843
GTGCCTC52150.06.4429470
AGTGTGC67400.06.387228568
CACCCCC10501.361348E-66.33332156
TCACCCC13701.04331775E-75.87590155
TGTGCCT62300.05.73032668
TGCCTCT51850.05.535187270
GCGTGCG7650.0090840445.490185346
GTAGGGA162900.05.37138370
GAGTGTG86150.05.240848570
TGTAGGG180400.05.180145770
GGGTGAC17601.040753E-75.170444526
AGGGGGG19708.767529E-95.1528141
AGAGTGT106150.05.011766470
Produced by FastQC (version 0.11.5)