FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_C24SPL16_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_C24SPL16_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199524
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6480.32477295964395264No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGACT150.00222220370.020
TAGTCCG452.6884663E-854.44444711
CGGACTA452.6884663E-854.4444476
CCGGACT200.006943661352.55
TGGCACG200.006943661352.516
GCTCCGC200.006943661352.526
CGGACTG506.16692E-849.0000046
GACTAGT451.6787435E-646.6666688
GGACTAG503.4663917E-642.0000047
TCGGACT503.4663917E-642.0000045
GACGGAC350.001253276140.04
CTAGTCC556.6714274E-638.18181610
GACTGTA708.652096E-735.08
ACTGTAG851.0575059E-732.9411779
GGACTGT751.4836642E-632.6666687
CAGTCCG500.007196316528.00000227
AGTAAGG500.007196316528.00000221
ACGGACT1056.729606E-726.6666665
CTGTAGA1101.0098665E-625.45454410
ATCTCGG700.001223003625.070
Produced by FastQC (version 0.11.5)