FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_C24SPL15m2_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_C24SPL15m2_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598525
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10450.17459588154212438No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCTG500.007207692627.9999688
AGTTCCC1000.00968051417.5014421
AGACTCA4451.887336E-48.65167538
ATTAGTG6151.2337368E-68.53657569
AGTACGT4500.00200595787.77841851
CGTGTAT4150.0084580837.590351658
AGAGTGT12152.0008883E-117.48970370
AAGACTC5159.0732507E-47.475719537
GATTAGT6351.4486138E-47.16534668
GAGTGTT10805.8826117E-97.129620669
TTTGACT5550.00200556576.936928323
AAGAGTG14008.0035534E-116.74999269
AGATTAG5800.00318806436.63792365
GTTAAGA8601.9639412E-56.511619668
AGTGTTA10803.1771742E-76.481473470
TGTTAAG8702.3274059E-56.43677467
TTAGTGT7953.303863E-46.16351570
GTGTTAA10302.6772163E-45.43688766
AAAGAGT18702.3683242E-95.42780170
AAGATTA9757.530734E-45.384608768
Produced by FastQC (version 0.11.5)