FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_C24SPL15m1_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_C24SPL15m1_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468680
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10290.21955278654945806No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG703.4864803E-530.06
GGACTGT1107.46809E-419.0909087
ATAGTCA1350.002943123215.55555563
GACTGTA1450.004734351814.48275858
CCTGTGT2600.001592427110.76923169
GTACTGC2300.00787697610.6521741
CTGTGTA2650.00186626510.56603870
GCCTGTG2850.00341363259.82456269
TCGCCTG3100.00682655959.03225970
CTTCGCC4753.812952E-48.10526469
TACTGTT3900.00484162488.0769232
GAGTGTC8302.2053428E-88.01204967
TCTTCGC5351.5811788E-47.85046770
AGTGTCT8251.6942613E-77.63636468
GTCTTCG5200.00100445517.40384667
GTGTCTT9502.3752546E-77.000000569
GTAGATC6157.8430003E-46.82926870
GTACTGT7055.197797E-46.45390031
AGAGTGT10954.0614395E-76.392694566
AAGAGTG12202.804578E-65.73770565
Produced by FastQC (version 0.11.5)