FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_C24SPL14_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_C24SPL14_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90284
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5270.5837136148154712No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGCA200.006931401352.526
CCTCCGC200.006931401352.537
GATGGCC200.006931401352.549
CGCACAC200.006931401352.529
CGGACTG305.850494E-446.6666686
GCTAACT350.001249631440.069
ACGGACT556.630895E-638.1818165
CCATGAT400.002407679635.010
ACTGTAG400.002407679635.09
TGATGTA450.00428765931.11110941
CAGAGCC1400.003718344715.066
AAGAGTG3802.7038586E-611.05263164
GTGTTCA2650.001841215210.56603868
TGTTCAG2350.00914216510.42553269
GAGTGTT3400.00137363999.26470666
AAAGAGT4209.816807E-59.16666663
AGAGTGT3500.0017890799.065
GAAAGAG4753.7252373E-48.10526362
GGAAAGA4753.7252373E-48.10526361
AGGGAAA4750.00334322037.368420659
Produced by FastQC (version 0.11.5)