FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n02_C24SPL11_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n02_C24SPL11_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143831
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8200.5701135360249182No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCTA150.00222093770.000012
TTACGTC150.00222093770.0000125
TGGTCGC207.905088E-570.0000155
GGTCGCC252.3839844E-456.00000456
CGCCGTA252.3839844E-456.00000459
CGGCGCG200.00693973652.50000413
GGACTGT608.367351E-1152.5000047
GTAACCC200.00693973652.50000429
CGGACTA200.00693973652.5000046
ACAGTCC506.150731E-849.00000426
ACTGTAG751.4551915E-1146.6666689
CAGTCCG451.6754475E-646.66666827
CGGACTG703.8016879E-1045.0000046
GACTGTA802.910383E-1143.7500048
GTCGCCG400.002412424735.00000457
CTGTAGA1055.4205884E-1033.33333610
GTAGAAG957.546987E-933.15789812
TCTACAG901.7406273E-731.11111323
GCCGTAT450.004296057431.11111360
TGTCCTA450.004296057431.11111332
Produced by FastQC (version 0.11.5)