FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11480254
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1766841.539025181847022No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG237330.20672887551094252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT35150.028.2120294
AGGGGGG32850.022.4421161
GCCGTCT55300.018.5592386
GCTTGAA51100.017.43251816
TGCCGTC67500.015.35893255
TATGCCG67100.014.8304023
GACTGTA21750.014.5004928
GCGGACT7750.013.4218735
CTGCTTG76800.012.32104214
TCTTCTG85150.012.29265210
CTTGAAA75050.012.28430117
CCGGACT8750.011.8879455
CGGACTA9450.011.3742696
TGGGGGG166200.011.1940191
GCCCGAC5804.6020432E-1010.7921471
GGGCCAC6008.3127816E-1010.4324081
TGCTTGA91750.010.35119515
CGTCTTC106600.09.9161538
ACGGGGG7101.3278623E-109.85165731
GTATGCC106350.09.6518852
Produced by FastQC (version 0.11.5)