FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_pIXSBP_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_pIXSBP_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2622761
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4650.038.3911746
TATGCCG4900.034.29066548
TGCCGTC4850.033.2006750
ATGCCGT4900.031.4331149
CGTATGC5500.031.18572446
GTATGCC6650.026.3195947
GCCGTCT4500.025.67086451
GGACTGT7100.024.152897
CAGTCCG4750.022.84017827
GACTGTA7150.022.0261148
ATGCCGG1901.3546014E-820.26608349
GCGGACT2653.6379788E-1219.8134255
TCGGACT4350.019.3123975
TGCCGGC2002.4418114E-819.2527850
CTCGTAT8750.019.2002144
TCGTATG9650.018.49866945
ACTGTAG8800.018.293919
ACAGTCC5800.018.1019226
CTGCGTG3053.8198777E-1117.215659
CGGACTA2852.6739144E-1017.1948096
Produced by FastQC (version 0.11.5)