FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_pIXHALO_SBP-bZIP53_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_pIXHALO_SBP-bZIP53_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3892340
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7350.032.38394548
TGCCGTC6000.029.16899150
GTATGCC9050.027.07432747
ATGCCGT7400.026.01558549
CGTATGC8550.025.79185946
CGGACTG4450.025.1737796
GCCGTCT5850.022.73685351
TATCGCG5800.019.3096541
TGCCGGC3100.019.19507850
CTCGTAT11750.017.57541744
GGACTGT6300.016.665217
GCGTGAA7100.016.26827461
CGTATCT19350.016.09719839
TCGTATC28950.015.83667538
CTGCGTG3905.456968E-1215.25762759
CAGTCCG3957.2759576E-1215.061975527
TCTCGTA14300.014.68610743
TCGTATG13650.014.61617445
CGTATCG10750.014.324697539
TATCTCG16000.014.21823841
Produced by FastQC (version 0.11.5)