FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_WRKY18_col_B_MM9430_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_WRKY18_col_B_MM9430_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5961815
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG5900.020.765256
CAGTCCG6050.013.30439727
GGACTGT10850.010.9665977
AGGGGGG3652.160376E-510.550651
ACTGTAG13350.09.9615079
ACAGTCC10800.09.07312826
CTACAGT11950.08.19998224
GACTGTA16000.08.0929058
TGGGGGG4400.00161023827.95658451
TGCCGTC6603.402929E-67.955783450
GCCGTCT7603.777859E-77.83023251
TATGCCG7251.5547139E-67.725340448
CTGTAGA17750.07.68933810
ACCGGGG5057.397111E-47.62296770
CGTATGC7957.7349614E-77.48537946
GTATGCC7552.8585728E-67.418311647
CCGGACT9201.3719728E-77.2291445
GGCGAGG5854.4490505E-47.18036460
ATGCCGT7855.115444E-67.134868649
GAACTCC102600.06.92422970
Produced by FastQC (version 0.11.5)