FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_SG163_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_SG163_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3076936
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT31820.10341456565882423TruSeq Adapter, Index 13 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG6250.038.084056
TATGCCG8300.034.1602648
GCGGGGT3100.031.61677752
ATGCCGT8350.031.44046849
CGTATGC9550.031.15514646
TGCCGTC7600.030.39796650
GGACTGT7850.029.8704477
GCCGTCT5950.029.41489251
CGGGGTG3850.029.0954253
GTATGCC10350.029.08521847
CTGCGTG3550.025.63694659
GACTGTA9350.021.7096588
CTCGTAT11050.020.9058444
TCGTATG12300.020.77389145
AATCGCG6250.020.71883641
TGCGGGG2201.5643309E-1020.68401551
CAGTCCG5700.020.2616927
TGCCGGG2302.8376235E-1019.78471450
CCGGGGT1951.8266292E-819.7460152
ATCGCGT4850.019.48375942
Produced by FastQC (version 0.11.5)