FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_SBP-bZIP53_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_SBP-bZIP53_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13469706
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18650.08.82112248
GTATGCC25900.07.43300747
TGTAGCC103050.07.06476261
GGTGTAG112850.06.60689459
GTGTAGC108750.06.565829360
CATTCAG132100.06.040402470
GTCACAT217200.05.994003370
CATGGTG133800.05.938756556
CGGGGTG10651.1481781E-55.916345653
TCCAGTC279350.05.87567870
TGGTGTA140050.05.798678458
CGTATGC28400.05.792679346
CTCGTAT31700.05.7414744
AGTCATA300700.05.68216371
CAGTCAC241150.05.65995369
CTCCAGT290000.05.647831470
CACTAAC181050.05.644405421
ACTCCAG312650.05.63045470
CCAGTCA278300.05.62118970
TCATGGT152400.05.604398755
Produced by FastQC (version 0.11.5)