FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_SBP-ColSPL16_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_SBP-ColSPL16_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091504
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4300.047.218456
GGACTGT6650.028.9453627
CCGGACT3600.026.2550835
TAGTCCG3000.025.66488611
CGGACTA3150.025.5605056
GACTGTA6950.025.1781128
ACTGTAG7400.025.065839
TCGGACT3800.023.0307755
TATGCCG4350.022.52986748
CTGTAGA8450.022.36531410
TGCCGTC3800.022.10637350
GCGGACT2700.022.0413045
CACGGAC1957.7852746E-1021.5426334
ATGCCGT4250.021.41283849
TGCCGGG1356.0259335E-620.74178150
CTAGTCC3550.020.70279110
CAGTCCG4400.020.68038427
CGTATGC5150.020.38937246
GCCGTCT3500.020.00148251
GTATGCC5700.019.6501147
Produced by FastQC (version 0.11.5)