FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_SBP-C24SPL16_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_SBP-C24SPL16_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115942
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG3050.050.5070656
GGACTGT3450.041.5899967
GACTGTA3300.037.117468
ACTGTAG3900.031.4070829
TATGCCG2450.030.00368948
CCGGACT2000.029.7589915
TGCCGTC2600.028.27270750
ATGCCGT2650.027.73925849
CGGACTA1800.027.2304526
GCCGTCT2100.026.6711451
TCGGACT2350.025.3268035
GTATGCC2950.024.91831847
CTGTAGA5150.024.46354510
GCGGACT1451.7236744E-824.1452265
TAGTCCG2051.8189894E-1223.90002311
CCCGGAC901.9248771E-423.3403874
TGCCGGC1052.038619E-523.33620350
CAGTCCG2550.023.33097327
CGTATGC2750.022.91190546
GGACTAG2301.2732926E-1121.3021937
Produced by FastQC (version 0.11.5)