FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_SBP-C24SPL14_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_SBP-C24SPL14_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086227
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4150.043.8619736
GGACTGT4150.043.0105487
TAGTCCG1900.038.6828611
GACTGTA4100.037.559678
ACTGTAG4300.035.8127069
TCGGACT3100.035.0052265
CGGACTA2350.032.7708476
CTAGTCC2350.031.27550510
GGACTAG2450.029.9989537
CCGGACT1700.028.8278335
CTGTAGA5500.027.36268210
CAGTCCG2800.026.24908627
ACTAGTC3000.025.6657719
TACAGTC3650.023.01289625
GCGGACT1854.0381565E-1022.7060935
GACTAGT3250.021.537718
ACAGTCC3500.020.9992726
GTAGAAG7000.020.9992712
TGCCGTC1356.023576E-620.74097350
TCCGGAC850.00378735520.5913094
Produced by FastQC (version 0.11.5)