FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_SBP-C24SPL11_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_SBP-C24SPL11_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804293
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG2500.042.00826
CGTATGC3350.027.16571646
ATGCCGT2750.026.72878849
TATGCCG3200.026.25148648
GGACTGT3550.025.63177
GTATGCC3800.024.8698347
TGCCGTC3350.022.98637650
CAGTCCG1751.2675991E-719.9983627
GCCGTCT3250.018.30923751
GACTGTA4500.017.8874238
CGCGTAT1200.001340960617.50099244
CTCGTAT4700.017.1286344
ACTGTAG5150.016.3093439
CCGGACT2251.7606435E-615.5585935
GCGGGGT1350.002945818515.55686852
CGGGGTG1605.160841E-415.3137953
TCGGACT2901.05465006E-714.4855855
TCGTATG5650.014.24859445
CTGTAGA6000.013.99885210
GCGGACT1550.007367040513.5510315
Produced by FastQC (version 0.11.5)