FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_HALO-bZIP9_SBP-bZIP53_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_HALO-bZIP9_SBP-bZIP53_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14685918
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29550.020.3741948
GTATGCC36900.016.97987747
ATGCCGT35100.014.75918949
AGGGGGG17500.013.8040391
TCGTATC109700.013.49511438
CGTATGC45050.013.28645746
TATCGCG23800.013.08756441
CGTATCT75150.012.71383239
TTCGTAT126400.012.54278537
CTCGTAT47000.011.9898944
GCCGTCT31400.011.81669151
TGCCGTC41400.011.75232650
ATTCGTA136100.011.70027536
TATCTCG65050.011.35222341
TCGTATG46500.011.29108945
CGTATCG46200.011.13571739
ATCGGGG12050.010.7466342
TGGGGGG17800.010.6210861
GTATCTC74700.010.307340
AATTCGT163050.09.89502835
Produced by FastQC (version 0.11.5)