FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_HALO-bZIP63_SBP-bZIP53_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_HALO-bZIP63_SBP-bZIP53_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18010881
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27400.016.09711648
GTATGCC38000.012.15954347
ATGCCGT36150.011.61983249
TCGTATC99500.011.15003738
CGTATCT72650.010.21269539
ATTCGTA117050.010.19578536
TTCGTAT115950.010.08128437
CGTATCG41000.010.07252339
TGCCGTC39050.09.95013250
CGTATGC42700.09.91929446
TGGGGGG16450.09.7901661
CTCGTAT51650.08.60635644
AATTCGT144100.08.5732635
TATCTCG68800.08.49511641
GAATTCG144150.08.47314934
TATCGCG23100.08.33282141
TCGTATG48100.08.22315745
ATCGGGG13500.08.03685542
GCCGTCT33850.07.96278551
CGAATTC154400.07.819961533
Produced by FastQC (version 0.11.5)