FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_HALO-bZIP53_pIXSBP_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_HALO-bZIP53_pIXSBP_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8774668
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13450.014.57397148
GTATGCC16850.011.84096647
TATCGCG10350.011.83519841
AGGGGGG9300.010.917141
TCGTATC46750.010.40587338
CGTATGC19600.010.00095946
ATTCGTA55050.09.98131336
CGTATCG18400.09.89078739
GAATTCG63550.09.25196834
TTCGTAT52450.09.14156237
ATGCCGT16150.09.10298849
CGAATTC69850.08.76823133
CGTATCT35500.08.67560339
ATGCCGG10500.08.66744849
TATCTCG32750.08.44235541
AATTCGT68250.08.30716335
TCGTATG22850.07.81238745
TGTAGCC57650.07.710621461
CTCGTAT25450.07.701762744
TGCCGTC20500.07.6836250
Produced by FastQC (version 0.11.5)