FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_HALO-bZIP25_SBP-bZIP53_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_HALO-bZIP25_SBP-bZIP53_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5870404
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG9100.034.6197056
TATGCCG10600.027.0783948
CGTATGC11400.024.87110346
TGCCGTC8350.024.73320650
GTATGCC11750.024.72606847
ATGCCGT9950.023.21838649
GCCGTCT7250.022.20967151
GGACTGT13450.021.8569987
TATCGCG7300.021.0949341
GACTGTA14750.018.5070028
CAGTCCG7600.017.95909727
TCGTATC39400.017.49905638
CGTATCG15500.017.16036439
ACTGTAG16150.016.902689
ATCGCGT7800.016.15338542
CGCGTAT6400.015.86054444
GTATCGC11250.014.93239940
CTCGTAT17950.014.82003544
CCGGACT8900.014.5523795
TCGCGTA7450.014.09487943
Produced by FastQC (version 0.11.5)