FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_HALO-bZIP10_SBP-bZIP53_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_HALO-bZIP10_SBP-bZIP53_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13476933
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25700.022.33682648
TGCCGTC29800.018.55873550
GTATGCC34050.018.09282147
ATGCCGT30350.017.29975949
TATCGCG18000.016.1380241
CGTATGC35750.015.8616446
GCCGTCT26350.015.54238151
TCGTATC93850.015.28930738
CTCGTAT43600.013.72759844
CGTATCG37750.013.72091139
CGTATCT69450.013.50519139
TATCTCG57350.013.2425341
ATTCGTA122000.013.10975536
TTCGTAT116600.013.02654937
TCGTATG45600.011.82098645
GTATCTC70050.011.54104840
AATTCGT151300.010.91783335
ATCGCGT21500.010.906672542
AGGGGGG14600.010.79157451
CGGGGTG9850.010.66134253
Produced by FastQC (version 0.11.5)