FastQCFastQC Report
Fri 24 Jan 2020
H2F7JAFX2_n01_HALO-CCA1_SBP-LHY1_col_B_n.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2F7JAFX2_n01_HALO-CCA1_SBP-LHY1_col_B_n.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9368667
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT141590.1511314256339776TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32100.040.78263548
ATGCCGT29650.038.8400649
TGCCGTC27750.038.7241450
GTATGCC36750.037.05069447
CGTATGC34600.035.71105646
GCCGTCT22200.035.63453751
CTCGTAT43050.028.781844
ATGCCGG12050.028.7578849
TATCGCG23000.027.23746541
TGCCGGC10450.027.13158250
GCGTGAA20400.025.56475461
CTGCGTG11900.025.00367559
TATCTCG63150.024.88367341
TCTCGTA50700.024.5077643
CGTATCT77650.024.20315639
TCGTATC123250.024.16472238
GTATCGC27950.023.66568840
AGGGGGG15900.023.5617031
CGTATCG46850.023.23217839
TCGTATG49450.023.07548145
Produced by FastQC (version 0.11.5)